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Trial 1b estimated the digestion of the diets (employed chromic oxide as an indigestible digesta flow marker) using a subset of 5 weaned calves per treatment between d 52 and 56.
DNA was extracted from one member of this bird group, Streptopelia orientalis (S. orientalis, oriental turtle dove), and used to identify a female-specific DNA marker using a random amplified polymorphic DNA (RAPD) fingerprinting.
We have compared a real-time quantitative PCR for non human leukocyte antigen (HLA) target (insertion/deletion marker) using a commercial kit (AlleleSEQR Chimerism assay), and its microchimerism detection sensitivity (around 0.1%) was 1 step (10 times) lower than that of nested PCR-SSP or -SSCP methods for HLA-DRB1 alleles.
Immunohistochemical staining was performed for annexin-5 apoptosis marker using a rabbit polyclonal antibody (Abcam, Cambridge, UK).
VEGF was co-expressed with GFP and a puromycin resistance marker using a 2A-mediated expression system[40].
Both the intraretinal and subretinal donor cell populations were immunopositive for nestin, a neural stem and progenitor cell marker, using a human-specific antibody.
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Korkalo deleted an AR marker using an inpainting method [68], and this was a pioneering work in this DR area [21].
Genome coverage was estimated to be 2713.5 cM (90%) for 406 markers using a granddaughter design.
Genetic association of the trait was sought with >5000 gene-based mapped SNP markers using a Mixed Linear Model approach.
After ex vivo CT scan of the localizer, the pathologic slices were cut perpendicular to the urethra and along the localizer markers using a customized cutting device.
The experimental design provided an effective tool to allow discovery of significant markers using a non-parametric analysis of variance.
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