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Structural abnormalities, including double minutes, that were present originally were maintained in the xenografts, and acquisition of new marker types was rare.
The number of overlapping segments between marker types was determined as well as those segments unique for a marker.
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In this review, the principles, potential power, requirements, advantages, and disadvantages of the various marker types are discussed, along with their applications in a variety of aquaculture studies.
All missing marker types were assigned to either the common parent genotype, or the non common parent genotype through an imputation algorithm that considers linkage relationship between markers within each family [40].
No other marker types are capable of enabling this.
As an additional precaution, populations of opposite Ara marker types were grown adjacent to one another.
Several marker types were used to characterize cryptic species in PAC such as single-copy RFLPs, microsatellite markers and sequence markers [ 14, 15].
Although these may represent regions that cannot be addressed genetically using DArT markers, they may also represent regions where other marker types are disproportionately frequent.
Long stretches on the map without certain marker types are likely due to not enough heterozygous markers genotyped in both parents.
DNA fragments of all marker types were recorded in each individual as 1 for presence, 0 for absence or as missing value in unclear cases.
Thus, despite different marker types being used, these results agree well in regard to computed map lengths for the triticale genome.
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