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This system will be useful for simple construction of unmarked mutations or repeated use of the same antibiotic marker to generate multiple mutants.
SYTOX-Orange binds to the DNA of permeabilised parasites and is therefore used as a marker to generate death curves in the cytotoxicity assay (life-dead assay).
URA was used as selection marker to generate pCUP-GAFQ-GFP for visualizing GAFQ-GFP fusions using confocal microscopy.
The gene was subsequently cloned into pMIL, a derivative of pMig [ 28] that expresses a luciferase marker, to generate Neu-pMIL, in which expression of Neu is driven by the murine stem cell virus (MSCV) promoter.
Second, we hypothesized that an scFv can be fused to a protein with specific binding toward a cell surface marker to generate a heterobivalent molecule such that the binding of both is retained.
Strains UCC1111 and MPY1 (UCC1111 hat1Δ::HIS3) were transformed with a PCR product that contains the orc5-1 alinkedlinked to the natMX4 resistance marker to generate AP121 (orc5-1::nandX4) AP123P123 (orc5-1::nAP123 hAP123:HIS3).
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The six beads provided reference points in both imaging modalities and were used as markers to generate a spatial transformation matrix.
The 94 seedlings were analysed with markers to generate data for linkage map construction.
This study employed both dominant (AFLP) and co-dominant (RFLP and SSR) markers to generate a linkage map.
We performed multivariate analysis on the entire set of identified markers to generate a four-gene classifier that, in exploratory analysis, performs better than EREG/AREG.
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