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The study described herein was performed to determine whether there is any relation between MIA/CD-RAP levels and the degree of chondrocyte differentiation in tissue culture and to analyse whether MIA/CD-RAP may serve as a useful marker to control chondrocyte differentiation in in vitro tissue engineering.
Transcript levels were compared with levels of KRT8, an epithelial cell-specific marker, to control for the variation in stromal and inflammatory cell components between dysplastic and normal epithelium (Caldwell et al, 2004, 2006).
The transient expression of GmNAC6 was driven by the 35S promoter in soybean protoplasts and was measured by RT-PCR, relative to a helicase marker to control for any variation in the transformation efficiency.
85 A useful marker to control for this is the co-migration of a size marker or, alternatively, comparison of the patterns in question with the results from an actual partial digest (fragments resulting from an incomplete EcoRI digest have around 6.5, 9, and 10 kb, and a 4.1-kb fragment appears also in EagI- EcoRI incomplete digestion when using StB12.3 or equivalent probe).
85 A useful marker to control for this is the co-migration of a size marker or, alternatively, comparison of the patterns in question with the results from an actual partial digest (fragments resulting from an incomplete Eco RI digest have around 6.5, 9, and 10 kb, and a 4.1-kb fragment appears also in Eag I- Eco RI incomplete digestion when using StB12.3 or equivalent probe).
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We considered M = 10 markers to control the genome-wide false positive rate at α = 0.05 with the Bonferroni correction.
A BFDR-based decision rule can be derived to judge significant markers to control BFDR under a certain threshold α (α=0.05) so that the multiplicity adjustments are achieved.
These contradictory results highlight the need for further tests of the general hypothesis, using a broader range of aging markers to control for anomalous indicators.
The use of functional genomics approaches would contribute with the identification of molecular signatures associated with abnormal ovarian development or premature ovarian regression in cultured fish species, thus providing potentially useful markers to control sexual maturation.
The Bayesian approach takes into account the effect of population structure, using a covariance matrix based on neutral markers to control for demographic effects when testing for correlations between environmental and genetic differentiation [ 73].
Understanding the basis of such aberrant chondrocyte responses and whether resident progenitor cells are involved will be vital for the development of therapies and diagnostic markers to control and prevent OA progression.
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