Exact(6)
This locus was later chosen as a cell-autonomous marker to construct a fate map of the shoot apical meristem of mature seeds [52], [53] and to measure genomic instability in radiation-sensitive (uvh1) plants [54].
Moreover, using a single type of DNA marker to construct a lotus linkage map would easily lead to uneven marker distribution and large intervals between adjacent markers.
Briefly, strains were first P1 transduced for attR (linked to a kanR marker) and attL (linked to a cmR marker) to construct the non-inverted parental strain.
The predicted probability of distinguishing of DHAS and LDSDS was used as a surrogate marker to construct a receiver operating characteristic (ROC) curve.
Gel purified fragments were then cloned into the SalI- MluI sites of vector YEp112SpGAL [ 45], a 2 μm multi-copyplasmid containing a TRP1 selectable marker to construct YEp112SpGAL-WRNor WRN variants under the control ofa gal-inducible promotor.
The A. tumefaciens binary vector pC-HYG (Motteram et al., 2009) was modified by addition of the S. cerevisiae 2μ origin of replication and the URA3 selection marker to construct ternary vector pC-HYG-YR (Addgene ID – 61765) (see supplementary materials and methods).
Similar(54)
The families were genotyped with 150 markers to construct a 2764 cM (Haldane) male linkage map.
A computer used data on the location of the markers to construct an avatar of each man (see videos).
GBS method is a useful and powerful approach to rapidly get lots of markers to construct genetic maps with high resolution.
The F2 mapping population of 94 individuals was genotyped at the loci tagged by the preselected anchor markers to construct a linkage map skeleton.
As NGS technologies emerge, the genotyping-by-sequencing (GBS) approach rapidly gets hundreds of thousands to millions of markers to construct the high-resolution genetic map, and generates sufficient information and coverage of plant genomes (Edwards and Batley 2010).
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