Given that the plumage traits are likely controlled by relatively few genes [ 23], and that only a small fraction of the genome was surveyed with our AFLP markers, the lack of congruence is not surprising and more exhaustive genome-wide surveying and examination of pigmentation-related candidate genes (currently underway) will be needed to identify those loci.
A limitation of the 16S marker was the lack of resolution in the species separation of related flatfish (P. platessa and P. flesus) and gurnard species (C. lucernus, E. gurnardus, and T. lastoviza).
The inherently higher variability of HC3 binding decreases the likelihood of detecting significant differences of the magnitude of those found for the other cholinergic markers; nevertheless, the lack of significance for the HC3 marker was statistically distinguishable from the decrement in ChAT (p < 0.05 for the treatment × measure interaction).
Mature circulating endothelial cells, which are generally recognized as cells expressing endothelial markers (CD 144, CD 146, vWF, VEGFR-2) in the absence of hematopoietic (CD 14, CD 45) and progenitor (CD 34, CD 133) markers (despite the lack of a clear concensus on their phenotypic definitions)[25, 26], were excluded with this method.
The downside of these markers is the lack of specificity.
The large distance between microsatellite markers and the lack of heterozygosity in some of them hampered a fine mapping of the precise extension of identified deletions.
A limitation of several recently proposed infection markers is the lack of larger cohorts providing populational serum concentration measurements in order to establish normal reference values.
However, the large distance between microsatellite markers and the lack of heterozygosity in some cases make us to believe that MMP analysis is underestimating deletions equal or smaller to ∼100 kb.
Sex-specific effects associated with proinflammatory markers and the lack of significant associations with MRI abnormalities ought to be considered in future studies on mechanisms of cognitive impairment in metabolic syndrome.
Although Pandit et al. and our study agree on the observation of mesenchymal markers and the lack of a significant decrease in the miR-200 family, we did not observe a decrease in let-7d in the ILD patient samples in this study.
