This notwithstanding, attempts to use new marker technologies such as SNPs have already been carried out [ 26].
In this context, we are also working towards integrating DArT markers with other high-throughput marker technologies such as SNP [ 14].
Several molecular marker technologies such as, RFLP, RAPD, DAF, SSR, SSLP, AFLP, CAPS, SNP have been discovered for molecular mapping experiments [ 1- 6].
Approaches to the use of data from molecular markers in genetic evaluation for predicting breeding values have undergone considerable development as dense genome-wide marker technologies, such as high-density, high-throughput SNP chips, have become available.
Highly multiplexed, hybridization-based marker technologies such as SFP, DArT and SNP have the potential to further 'collapse' the vector of genotypic differences between bulks into a single (perhaps replicated) whole-genome assay [ 2- 5].
Several barley consensus maps have been built with gel-based marker technologies such as Restriction Fragment Length Polymorphism (RFLP), Simple Sequence Repeats (SSR) and Amplified Restriction Fragment Length Polymorphism (AFLP) [ 7- 11].
Due to the insufficient throughput, accuracy and data standardization, the existing molecular marker-based technology, such as SSR marker fingerprinting, is of limited use.
Such loci are a possibility in any marker technology when applied to a polyploid species such as oat, and they are almost certainly present to some degree in this study.
The extremely dense genetic markers derived from new genotyping technology, such as genotyping by sequencing, have provided the potential for discovering genes underlying phenotypic diversity through GWAS [ 3- 5].
Thirty six pairs of markers were polymorphic (△Tm > 0.3 °C) using HRM technology, such that ~65%% of these markers can be genotyped using this technology (Fig. 2).
