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This marker system has the ability to amplify DNA from dispersed polymorphic loci and has its power to detect small genetic differences [10].
To date, this marker system has delivered a number of applications that have advanced both sugarcane research and breeding.
This PCR-based, codominant marker system has remarkably increased the efficiency of transferring genetic information across species.
The high PIC value in N. crassa implies that the SSR marker system has sufficient resolution/polymorphism to be used for genetic studies.
A full-length gene-cloning method based on this marker system has been used to clone 16 or 17 LMW-GS genes in individual bread wheat genotypes [ 15].
The estimated PIC value was comparable and relatively high for SSR markers compared to other organisms [ 58- 60] where the SSR marker system has been applied to many genetic analyses.
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Numerous ecological applications for the SRAP marker system have been explored, although none have been pursued beyond the interests of crop development.
In recent years, the development of DNA marker systems has advanced our genetic diversity and phylogenetic understanding of plant genetic resources, but simple sequence repeat (SSR) markers were still relatively limited in the Orchidaceae family.
However, unavailability of genomic resources of co-dominant marker systems has been the major constraint for adopting molecular breeding to achieve genetic enhancement of Mulberry.
The utility of transposon-based marker systems has been widely proven in phylogenetic, genetic diversity, breeding, and mapping studies in various crop plants and tree species, due to their easy detection by a simple PCR [ 18].
The introduction of next-generation sequencing technology and the availability of inexpensive and high-throughput marker systems have made molecular breeding more attractive.
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