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To test these possibilities, we examined the presynaptic marker synaptobrevin using a synaptobrevin::GFP (SNB-1 GFP) SNB-1 GFP].
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We examined the subcellular localization of SNB-1 (synaptobrevin) using a transgene, juIs1[Punc-25::SNB-1::GFP], SNB-1 GFP]ses a SNB-1::GFP chimeric prothat in thexpressesic motor neurons [66].
To determine whether our newly identified rpm-1 mutations also impair presynaptic bouton formation, we examined the subcellular localization of SNB-1 (synaptobrevin) using juIs1, a transgene that expresses a SNB-1 GFP SNB-1 GFPsion in motorneurons.
To test the ability of the chimera to enter the nerve endings of central neurons, we added it to cultured hippocampal neurons and activated the neurons using 50 mM potassium chloride for 10 min. The presynaptic uptake of the clostridial chimera was assessed by colocalization with a synaptic vesicle marker, synaptobrevin.
We also examined fluorescence of the GFP-tagged synaptic vesicle marker synaptobrevin snb-1 expressed in a subset of the cholinergic motor neurons under the unc-4 promoter [27].
Synapses can be visualized in the 19 D type GABAergic motor neurons by driving expression of the synaptic vesicle marker synaptobrevin::GFP (SNB-1 GFP) SNB-1 GFPromoter of the C. elegans GAD (glutamic acid decarboxylase) gene, unc-25 [18], [19].
Nuclear markers were counted using ImageJ.
Markers were fastened using double-sided tape.
3) Figure 5: which synaptobrevin was used for colocalization with EGFP-Rab26?
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