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Using this marker strategy we showed previously that that CCL4 is made by CD8+ and CD4+ memory T cells where greater frequencies of CCL4+ cells were found for the CD8+ subsets [14].
Intuitively, an internal marker strategy has some attractive qualities, since it might sidestep any uncertainties about the relationship between confounding amplification and metrics of prediction of exposure.
In addition, with the use of the dual marker strategy, the diagnostic accuracy was high for the diagnosis of AMI at presentation (area under the curve: 0.97).
Hence, the proposed dual marker strategy is only reasonable in early presenters and when blood samples are drawn as early as possible.
Furthermore, by using a bin marker strategy, yield-associated loci in rice [ 36] and root-knot nematode resistance QTLs in soybean have been fine mapped [ 37], respectively.
Using re-sequencing technologies, Huang et al. [ 30] have developed an ultra-high-density linkage map by using a whole-genome re-sequencing and "bin marker" strategy.
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It has been reported that multimarker strategies including natriuretic peptides, troponin, and inflammatory markers are superior to single marker strategies [ 31, 32].
The rationale, design and methods of the "BIOmarkers associated with Sarcopenia and PHysical frailty in EldeRly pErsons" (BIOSPHERE) study are described as an application of a multi-marker strategy for the development of biomarkers for the newly operationalised Physical Frailty & Sarcopenia condition.
Compared with single marker tests, such as D-dimer, a multi-marker strategy may improve diagnostic ability.
Nevertheless, a multiple-marker strategy combining markers with independent clinical values may still benefit from these markers that increase the sensitivity but not the specificity for cancer detection[ 84].
Through use of a three-marker strategy, we have subdivided human breast tissue into Luminal 1 cells, characterized by the majority of cells having an EpCAMhiCD24+CD49f- profile; Luminal 2 cells, characterized by a majority of EpCAMhiCD24+CD49f+ cells; Basal cells, characterized by EpCAM+/loCD24-CD49f+ EpCAM+/loCD24-CD49f+al cells, chandcterized by EpCAMesenchymalf+ cells.
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