Protein molecular weight marker standards (GE healthcare) were used for column calibration and generation of standard curve to identify the molecular weights of the test protein sample.
The samples, molecular weight marker, and commercial standards for MMP-2 (Chemicon, Temecula, CA, USA), MMP-3 (Abcam, Inc., Cambridge, MA, USA), MMP-9 (R&D Systems, Minneapolis, MN, USA), MMP-14 (Chemicon), TIMP-1 (R&D Systems), and TIMP-3 (Abcam, Inc).
However, currently within the South African public health sector there are no uniformly agreed-upon quality markers and accreditation standards for our ECs.
For example, our results suggest that, for neutral genetic markers, standard poodles are as differentiated from black and brown medium sized poodles as from giant schnauzers or Siberian huskies (Table 2).
Of the 70,000 SNPs targeted for manufacture on the array, 6942 markers failed to meet standards for bead representation and decoding metrics during the array construction process at Illumina and were removed from the manifest.
Scoring standards for each marker are shown in supplementary material Fig. S3.
Although CD133, a 120 kDa cell-surface marker of normal human neural stem cells (NSCs), is not a specific marker or gold standard for identifying glioma stem cells, it has been used in most relevant studies for enriching tumor stem-like cells from brain tumors.
The protein marker, Mark12 Unstained Standard, for SDS-PAGE, was from Invitrogen Inc. (Carlsbad, CA; Catalog No. LC5677).
The U.S. Department of Transportation sets forth national standards for highway signs, road markers and other signals in its Manual on Uniform Traffic Control Devices.
