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Exact(7)
However, only single marker staining was shown.
Human ESC marker staining was performed after 20 passages.
B220 and Cd19 marker staining was performed as described above, but the GFP marker was not gated upon since GFP expression is extinguished upon removing dox.
For each marker, staining was graded independently by two observers (unaware of animal treatment) on a scale of 0 (no staining at all) to 3 (maximal staining).
Intensity of marker staining was not used as a method for scoring the tissue sections because the degree of antigen masking is dependent upon the fixative used, its degree of penetration and the fixation time.
Diffuse positive Ki67 (a cell proliferation marker) staining was detected in Smad7-mediated liver metastases, as compared to livers from parental FET and vector control cells, suggesting the involvement of increased cell proliferation in metastatic tumour growth.
Similar(53)
The cells with positive desmin (myogenic marker) staining were used for identification of myogenic differentiation.
Figure 4: Most differentiation marker staining is of poor quality and hard to interpret.
The cells were washed, and HLA tetramer and surface-marker staining was performed as above.
Immune markers staining were compared between TT and NT using paired t-test for quantitative variables and Bowker's test of symmetry for qualitative staining variables.
For three-way multichannel FACS with the transcription factor-GATA4, SOX17 and cell surface marker CXCR4, staining was performed as follows: After fixation and blocking, cells were labeled with mouse anti-human CXCR4-PE antibody.
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