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Cells were collected and stained with DRAQ5 live/dead marker solution.
Aliquots of 100 µL of the standards and samples were added to blank micropores and 50 µL enzyme marker solution was added.
The electrophoresis mobility marker solution (10 μL; preparation is described below) was added to each sample in the 96-well plate and dried in a CVE.
One microlitre from each PCR was combined with 4 μl formamide and 0.5 μl of a fluorescent size marker solution (Applied Biosystems, UK).
Consequently, uncertainty is less in the 012 allele coding system than in the 210 allele coding system because the more frequent homozygous allele multiplies the marker solution and uncertainty by zero.
Ten minutes after sedation, animals were intragastrically dosed (gastroduodenal feeding tube, Levin type; VEC Medical) with a marker solution containing 0.5 g mannitol (Sigma-Aldrich, Madrid, Spain) and 0.6 g Co-EDTA [ 14] dissolved in 15 mL deionized water for a total dose of 1.1 g of marker mixture per pig.
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In the present study, marker solutions were obtained according to three different methods that were chosen on the basis of their statistical properties, treatment of the marker effect and correction for population stratification.
There are several arguments supporting the full kinetic analysis: first, considering the relevant large volumes of marker solutions and the individually different states of the veins of the patients, the administration of the marker during a reasonable time – 3 minutes instead of a truly instantaneous bolus injection – is possible.
The nuclei of neurons were labeled by a fluorescent marker (Hoechst solution; Sigma-Aldrich).
We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution.
Methods in routine clinical use today such as radioactive seed localization, radio-guided occult lesion localization and wire-guided localization suffers from limitations that this injectable fiducial tissue marker offers solutions to.
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