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Immunostaining using antibody against phospho-histone H3, which serves as M phase marker, shows a significant increase of M phase cells in the wing discs of dRecQ414 larvae compared with that of wild type (Figure 6B).
We tested if the GFP marker shows a Mendelian segregation assuming a dominant marker on a single diploid locus.
This simple, highly repitable, inexpensive and easily available marker shows a potential to select patients at high risk for poor clinical outcome for appropriate treatment strategies.
However, the differences in the number and location of H3 histone genes in L. sinapis and especially L. juvernica are rather surprising, since this marker shows a highly conserved pattern in the lepidopteran family Tortricidae [ 52] and other insect groups, such as the Acrididae grasshoppers [ 53] and Scarabaeinae beetles [ 54].
A distribution of the number of positive BACs found per marker shows a two-zone distribution with about 79% of markers evenly distributed in the 1-6 BAC/marangerange while the remaining markers are linked on average to 9-10 BAC/marker, probably reflecting the fact that about 20% of the primers used amplified duplicated or closely related genomic sequences [Figure 1].
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Chromosome 8 was assumed to be abnormal if at least one marker shows an AR⩾1.4.
In contrast, H3K4Me2 another activation marker showed a strong punctuate distribution spread throughout the nuclei.
Staining for LC3 (green) as an autophagy marker showed a faint and diffuse cytosolic pattern in these cells (Figure S3).
The expression of otx2, a mid brain marker, showed a decrease in total abundance in the morphants, compared to the wild-type embryos (Figure 3A).
No targeted capture marker showed a perfect match to sex.
Interestingly we found IFABP-a-SNP1245 IFABP-a-SNP1245 IFABP-a-SNP1245ociation to the traits.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com