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Exact(6)
Primer pairs targeting the SCAR marker sequence were designed, which enabled species-specific detection and avoided cross-amplification of other species of Serranidae fish.
If none of the results returned for a marker sequence were supported, then the top hit was retained, despite the lack of support.
Together the MATα locus and LEU2 marker sequence were amplified using PCR and transformed into YPH278 to replace the SUP11 and URA3 sequence on the present mini-chromosome (MC).
Nevertheless, an interpretation using the strict criteria that no ChIP was observed for the B73 samples, no ChIP was observed for either of the 9Bic-1 replicates, but that ChIP-seq reads covering the entire marker sequence were observed for all three TB-9Sb replicates indicates that at least seven markers lie within the functional B centromere core.
The marker sequence were finally assigned to certain ancestral blocks according to the best match length, E value, good alignment consistency with adjacent loci, good alignment consistency among bin markers, good alignment consistency with other published genetic map of B. juncea.
The DArT-seq marker sequence were only 69 bp in length and therefore likely to generate a large number of alignments for the SY-PM genetic mapping population and the 26 accessions of the three species in which A, B and C genomes contained.
Similar(54)
High quality marker sequence was processed through a CUGI pipeline consisting of Repeat Masker [ 49] with the RepBase database [ 50], cross_match [ 51], and Tandem Repeat Finder [ 52].
In some instances, the best BLAST hit of the marker sequence was not on the homologous chromosome of P. trichocarpa and S. purpurea.
If a marker did not hit any EST, the marker sequence was used to search in wheat genome sequences to test if the marker hit any contig that contains a gene.
GBS07859 showed a match with contig1041452 (1,002 bp) of Chinese Spring genomic sequences (http://www.cerealsdb.uk.net) and a cDNA sequence of diploid wheat T. urartu UCW_Tu-k41_contig_833), suggesthat thet the GBS07859 marker sequence was amplified from a gene.
The highest scoring alignment for each marker sequence was identified, and was considered comparative only if all high scoring alignments for that marker consistently aligned with the same region of the human genome.
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