Relevant marker sections were amplified in a 25-µl multiplex PCR reaction including 1 µl sample DNA (35 cycles, Tm 55°C, 0·2 µl MyTaq™ enzyme).
(XLS 142 kb) Supplementary Table 5 Analysis of allelic variation between lines and Pokkali accessions using 61 background SSRs (section A) and 17 Saltol markers (section B). (XLS 37 kb) Supplementary Table 6 Markers in and around the Saltol region on the short arm of chromosome 1.
To investigate the heterogeneous expression of the newly identified broadly expressed RPC markers, section ISH and DISH were performed for one such gene, Fgf15.
For simplicity, we have included E-cadherin and Sox2 transcripts within the 'pluripotency markers' section of Figure 5B, although the former is expressed in differentiated epithelium and the latter can be expressed in ectodermal lineages [23], [25].
CC and PQ compiled the current biochemical markers section.
Why have some markers (section 4.1.2) been difficult to detect in the periarterial spaces?
Interestingly, the capacity of an MSC for homing seems to be related in part to its expression of Stro-1 (see the 'MSC markers' section above) [ 7].
The PCR program consisted of 94°C for 90 s; 40 cycles of 95°C for 30 s, 53°C for 1 min 30 s, and 72°C for 2 min; followed by final extension at 72°C for 5 min. The amplified fragments were separated on 1% (v/v) agarose gels in TAE buffer, and then photographed and analyzed as described in the REMAP markers section.
For double immunostainings of OX40 and T cell markers, sections were incubated with anti-OX40 (1:30, Pharmingen) overnight at 4°C, washed, further reacted with biotinylated donkey-anti-rabbit antibodies (1 500 Jackson ImmunoResearch, West Grove PA, USA) for 1 hour at RT, incubated with avidin-alkaline phosphatase (Sigma, Germany) and developed with Fast Blue (FB, Sigma, Germany) substrate.
In our model, all hidden nodes were connected, each of them featuring six emission states representing the observed alphabet of genotypes (see Genotyping at individual marker positions section above).
