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We blindly classified cells with the Pacific Blue channel turned off, and then compared our identifications with the marker protein patterns.
We evaluated cytoplasmic and nuclear expression of these proteins in differentiated, dysplastic, and cancer cells, compared expression of proteins, and discussed the potential clinical value of the differential expression of the studied marker protein patterns.
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We previously used proteomics analysis to compare SCC and squamous vaginal cancer (Hellman et al, 2004, 2009; Lomnytska et al, 2010), and we extend the analysis in a current study aiming to establish a marker protein pattern for objective detection of SCC precursor lesions.
Our study is the first to assess a marker proteins pattern previously identified by proteomics-based analysis of SCC for the changes in expression during the sequential steps of SCC carcinogenesis.
The similarities in marker protein expression, cell morphology, division patterns, and neurogenic scheme between hNPC cultures and in vivo neurogenic progenitors provide significant evidence that hNPCs utilize developmental mechanisms to generate neurons even in artificial in vitro environments.
It is unknown how the protein patterns of tumors change during metastasis or whether markers are present that allow metastases to be allocated to a specific tumor entity.
Open image in new window Fig. 1 Protein patterns of various salted shrimp pastes, LM low-molecular weight marker, key: see Table 1 caption.
Through the expression of a GFP-tagged exosomal surface marker protein by the cells of origin, the recipient plasma membrane exhibited fluorescing GFP patterns after exosomal uptake.
Cluster analysis of protein spectra was performed to identify protein patterns potentially related to established clinicopathological variables and/or tumor markers.
The aggresomes were recognized by the marker protein p62 (red).
Typical immunohistochemical staining patterns observed for the EMT marker proteins in NSCLC are shown in Figure 1(a).
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