We blindly classified cells with the Pacific Blue channel turned off, and then compared our identifications with the marker protein patterns.
We evaluated cytoplasmic and nuclear expression of these proteins in differentiated, dysplastic, and cancer cells, compared expression of proteins, and discussed the potential clinical value of the differential expression of the studied marker protein patterns.
Our study is the first to assess a marker proteins pattern previously identified by proteomics-based analysis of SCC for the changes in expression during the sequential steps of SCC carcinogenesis.
The similarities in marker protein expression, cell morphology, division patterns, and neurogenic scheme between hNPC cultures and in vivo neurogenic progenitors provide significant evidence that hNPCs utilize developmental mechanisms to generate neurons even in artificial in vitro environments.
Through the expression of a GFP-tagged exosomal surface marker protein by the cells of origin, the recipient plasma membrane exhibited fluorescing GFP patterns after exosomal uptake.
The aggresomes were recognized by the marker protein p62 (red).
Peroxisomal marker proteins were detectable in a segmented pattern on such elongated peroxisomes, like beads on string, with constriction of the membrane (Grabenbauer et al., 2000; Koch et al., 2004; Itoyama et al., 2012; Itoyama et al., 2013).
Typical immunohistochemical staining patterns observed for the EMT marker proteins in NSCLC are shown in Figure 1(a).
In this study, overall survival rates were analyzed in terms of the clinicopathological features of OSCCs as well as the immunohistochemical expression patterns of the three marker proteins.
Finally, we were interested to investigate marker proteins not only correlating with the disease stage, but also patterns of tissue damage.
