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To address this fundamental question, we first performed cancer stem cell marker profile analysis using human breast cancer cell lines and specimens to further define and characterize different stem cell populations by flow cytometry, along with in vitro and in vivo assays to verify the stem cell properties of different cell isolates.
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Cells were then subjected to stem cell marker profiling analysis.
The stem cell marker profiling analysis was performed using a BD FACS Canto II.
To investigate whether breast cancer cells freshly derived from clinical specimens express heterogeneous stem cell markers as observed in human breast cancer cell lines, we performed stem cell marker profiling by FACS analysis of nineteen specimens obtained from National Taiwan University Hospital.
Analysis of the cell surface marker profile showed spatiotemporal differences in VCAM-1 and endoglin expression.
Some of the selected markers listed above were also included in this profile analysis (IL-6, IL-8, ICAM-1, MMP-3, TNF-α, and VEGF).
Analysis by flow cytometry of the cell surface marker profile was conducted by harvesting ASCs with 0.25 % trypsin/1 mM EDTA for 3 4 minutes at 37 °C.
Analysis by flow cytometry of the cell surface marker profile was conducted by harvesting ASCs with 0.25% trypsin/1 mM EDTA for three to four minutes at 37°C.
This indicates that studies with higher marker densities have greater power to detect QTL regions by QTL intensity profile analysis.
Expression profile analysis in multiple human tumors has identified L1 as a molecular marker for differential diagnosis and targeted therapy [ 21, 22].
These results validate the importance of core-fucosylated proteins profile analysis in CRC as a way to discover new tumour markers or therapeutic targets.
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