Exact(2)
Sequence alignment of STS-PCR marker products of CS, Glu-A3a and its CDS.
The 19 PCR marker products larger than 300 bp, together with the products of 30 markers that produced ambiguous segregation patterns following HRM analysis, were sequenced directly, enabling a further 29 polymorphic markers to be mapped.
Similar(58)
The new Powerplex® S5 Mini STR-System from Promega with the four provided STR loci D18S51, D8S1179, TH01 and FGA as well as the Amelogenin marker (PCR products ranging from 80 to 220 bp not considering the longer FGA fragments) is designed as a screening tool especially in difficult casework samples.
To investigate whether the organ marker gene products are associated with specific biological processes, cellular components or molecular functions, we summarized GO annotation available for 16 nodule, 68 root, 75 leaf, 28 stem and 329 flower markers (Table 2).
If the marker gene products (mostly proteins) could be detected by antibodies using the extracts of leukocyte subpopulations in the PBMCs, the transciptome data would be practically useful for bedside diagnosis.
Quail gene marker PCR products were purified with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA, USA) and cycle sequencing reactions were performed in 10 μl with 10 to 20 ng DNA, 10 pmol of one of the PCR primers, and 2 μl of Big Dye Terminator Mix (Applied Biosystems, Foster City, CA, USA).
For dominant markers, PCR products were resolved in a 2% ethidium bromide-stained agarose gel in 1× TAE buffer.
The elimination of the marker gene product is desirable in the context of environmental safety [ 16, 20, 24, 46, 47].
All eighty-two sets of primer pairs from barley EST-SSR markers amplified products within the H. chilense genome.
For SSR and IDP markers, PCR products were analyzed electrophoretically on a 2% agarose gel or 6% polyacrylamide gel.
For SNP markers, PCR products were cloned into a pGEM-T vector and sequenced to determine sequence variation.
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