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Even after exclusion of errors and selection of markers with less than 2% error rates, the microsatellite linkage maps are not only inflated, but also exhibit different marker orders to the known sequence.
Comparisons to RBT chromosomes [ 21, 33] revealed that all linkage groups identified in the F1 hybrids were syntenic with and had similar marker orders to specific RBT chromosomes, and therefore we defined each specific linkage group with respect to its homologous RBT chromosome, except RYHyb20 (a chromosome fusion) and RYHyb28 (RBT sex chromosome homologue) (Table 1).
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The r for the linear fit of RG marker order to ML marker order ranges from 0.09 to 1.0, with 29 of 47 being 1.0 and 36 exceeding 0.75.
Nicotiana is the most distant from the other species but appears to differ from tomato by 10 inversions only, possibly because multiple sequential rearrangements shuffled the marker order to the extent that individual inversions can no longer be deciphered [ 11].
Integrating linkage and RH data into a single map will refine marker order to facilitate genomic sequencing and will also increase the efficiency of identifying genes associated with QTL.
Sex-specific maps were produced using the sex-average marker order to recalculate marker intervals based on 37,306 phase known and 130,179 phase unknown meiotic events for the male map, and 43,071 phase known and 129,665 phase unknown meiotic events for the female map.
The additional constraint of fixed marker order (equal to the simulated physical order) was used to create linkage maps with no ordering conflicts.
However, for regions with a high density of markers, precise marker order has to be taken with care as the marker positions were calculated according to the positions of common markers from different maps and not based on recombination fractions.
However, inconsistencies in marker order led to difficulties in integrating around one third of these QTLs.
Inconsistencies in chromosome lengths and marker order led to different chromosome genetic lengths.
MAPMAKER was used to confirm marker orders determined by MSTMap and to convert the recombination fractions to centiMorgans (cM) using the mapping function of Kosambi [ 78].
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