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Where there were discrepancies between the order calculated by MapDisto and that based on the genome sequence, we calculated map length based on the marker order from the genome sequence.
On Chromosome V, the MapDisto order between markers cntg-157-107424 and cntg-98-505170 (Table S1) yielded a map length of 21.3 cM for this region, whereas the marker order from the genome sequence yielded a length of 58.5 cM.
MergeMap takes into account marker order from individual maps and calculates a consensus marker order.
MergeMap calculates a consensus marker order based on the marker order from individual maps.
The predicted marker order from the chicken genome assembly was initially chosen as the most likely order, and alternative orders were tested using the FLIPS option.
The marker order from the merged map generated was used to check maps generated individually for the four contributing parents to identify suspicious double recombinants that would indicate potential errors in the marker order.
Similar(51)
While low coverage and high variance in sequencing resulted in a large number of missing values for some individuals, a novel method of imputation based on maximum likelihood marker ordering from initial marker segregation overcame the challenge of missing values, and made map construction computationally tractable.
In this study, we have left distorted markers in all analyses, discarding them only from the minority of framework maps in which they prevent a reasonable fit between observed and expected recombination rates or good agreement of marker orders from the regression and maximum likelihood mapping methods.
Then, a genetic linkage group was constructed based on recombination frequency and SNP ordering was done using the ordering algorithm of RECORD (without imposing marker order) coming from REcombination Counting and ORDering, proposed by van Os et al. (2005a, 2005b).
The marker order obtained from the third round of analysis was retained with the JOINMAP command "calculate map".
The marker order predicted from the chicken genome sequence was CK304956-DV952125-DV952809-DV955012, which was also the marker order that produced the highest likelihood in the mapping population.
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