Unexpectedly, we found that a marker of transit-amplifying progenitor cells, EGFr, was also expressed by a subset of neuroblasts.
This suggests that, unlike the endogenous gene, the transgene persists in neuroblasts and is not a specific marker of transit-amplifying progenitor cells.
Consistent with this view, Sod2−/− skin trended towards lower expression of Krt1 and Krt10, markers of transit amplifying cells, and we previously de-monstrated that senescent cells increase with age in the suprabasal layer [ 6].
By contrast, mRNA levels of keratins 1 (Krt1) and 10 (Krt10), markers of transit amplifying cells and early differentiated cells in the epidermis [ 52, 53], were somewhat lower in Sod2−/− skin, although this difference was not statistically significant.
The "Bristol" stool form scale was used to assess the quality of stools based on a semiquantitative scale, as a marker of colonic transit.
KRT19, which is expressed by both basal and luminal epithelial cells and is thought to be a marker of the transit-amplifying population [ 42], was slightly increased in G3, but decreased in G4 with respect to luminal cells.
Tcf15 acts as a marker of this transition state: it is rapidly upregulated as ESCs transit from a naive to a primed state, and is associated with a subpopulation of epiblast-primed Oct4+ Nanog/Klf4-low cells.
This study sought to evaluate microsphere destruction (utilising signal intensity as a marker of contrast concentration) during transit through an ECMO circuit.
The aim of this study was to quantify the degree of microsphere destruction, utilising signal intensity as a marker of contrast concentration, during transit through an ex vivo ECMO circuit using quantitative echocardiography.
The number of markers left in the different segments of the colon was counted, and the GITT was calculated based on the following formula: GITT = M + f × D D where M is the total number of markers left; D is the number of markers ingested each day; and f is the fraction of the daily markers selected for the provision of transit.
