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To evaluate cell proliferation, Ki-67, a marker of proliferating cells, was immunolabeled in transverse frozen sections of rat brain, at 7 days after MCAO (Anti-Ki-67, 1 : 300, Abcam).
Ki67 is a marker of proliferating cells.
Nestin is a marker of proliferating neurons [30], but stathmin1 and nestin were localized in different populations of neurons.
To ensure that Sb did not promote the expansion of the proliferative compartment, ki-67, a marker of proliferating cells, was immunolocalized.
In order to further characterise the association of H4R3me2a with the proliferative or post-mitotic state of the neural cells, cortices isolated from E15.5 embryos were co-immunolabelled with antibodies against H4R3me2a and Ki67, a marker of proliferating cells.
Finally, we analyzed the levels of TER-119 (erythroid-specific marker) and CD71 (transferrin receptor; a marker of proliferating erythroid precursors) in the marrow and spleen of Drf1−/−RhoB−/− and Drf1−/−RhoB+/− mice.
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Immunohistochemistry was performed on control, JEV and JEV+M brain sections for the markers of proliferating cells: Ki-67, and PCNA.
Surface markers of proliferating cells were identified by multi-color flow cytometry.
The gene expression markers of proliferating callus capacity to regenerate plants by somatic embryogenesis should make it possible to predict callus lines suitable to be used for multiplication.
Surface markers of proliferating cells were identified by multi-color flow cytometry with fluorochrome-labeled antibodies to CD3, CD4, CD8, CD28, CD45RO, CD25, CD212 (IL-12R β2), and CD49a.
Differentiated cultures were subjected to expression analysis by real-time PCR of two well-known markers of epidermal suprabasal layers: keratin 1 (KRT1) and involucrin (IVL) as well as markers of proliferating basal cells: keratin 14 (KRT14) and PCNA.
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