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This mESC line was modified to express GFP in Lhx6+ cells, a marker of newly postmitotic and mature MGE-derived cortical interneurons.
In order to verify that neurogenesis was reduced, doublecortine (Dcx) was used as a marker of newly born neurons [36], [37].
Moreover, double-labeling analyses with calcein, a marker of newly formed bone, showed that the bone formation rate was significantly decreased in Cthrc1-null mice (Figure 2H).
Once β-gal+ cells were identified (Figure S3), adjacent cryosections were stained with antibodies against β-gal, CD34, and αSMA as a marker of newly regenerated myofibers [37], [38], [39].
In contrast staining for αVβ3 was not an absolute marker of newly formed vessels.
It should be remarked that DCX does not represent a marker of newly generated neurons throughout the brain.
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The early endosome marker, EEA1, which has shown to be also a marker of newly-formed macropinosomes in cultured cells (Kerr et al., 2006; Merino-Trigo et al., 2004), was detected on the FITC-dextran labelled macropinosomes in peritoneal macrophages.
This is shown by the elevated levels of both markers of newly generated neuroblasts (DCX and PSA-NCAM) and neurons (NeuN) within the OBs and DG of aged s-Ink4/Arf/p53 mice.
IL-1 receptor type 1 (IL1RI) transduces signals from proinflammatory cytokines IL-1β and IL1α and can serve as a marker of a newly described highly inflammatory effector Th subset, Th17 [ 26].
Eosinopenia can be used as a diagnostic marker of sepsis in newly admitted critically ill patients.
The principal finding of this study is that the presence of early repolarization before anesthesia is a marker of increased risk of newly observed cardiac events one year after surgery in low risk surgical patients.
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