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Furthermore, this antibody, being a marker of mitotic cells, can be applied in multiparametric cytometric analysis to study H3 phosphorylation in relation to expression of other proteins critical for cell cycle progression or chromatin condensation during mitosis.
Using BrdU incorporation as a marker of mitotic activity in RAM, the authors demonstrate that the mutant displayed a lower level of BrdU at the QC site compared to the control.
P-H3 is a marker of mitotic chromosome condensation [42].
Tumors stained for Ki 67 (present during all active phases of the cell cycle: G1, S, G2, and mitosis) and of phospho-histone H3 (the specific marker of mitotic cells) showed no difference between untreated and diclofenac treated mice, Fig S1.
In order to determine if CYP26B1 is required for maintaining mitotic quiescence in male germ cells, E15.5 and E16.5 testes were sectioned and IF was performed with antibody to Ki67 (Figure 6B, E, H, K), which detects all phases of the cell cycle except for G0 and is a common marker of mitotic cells.
Phosphorylated serine 10 on histone H3 is a marker of mitotic cells.
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Telomeres, the protective nucleoprotein structures capping the ends of eukaryotic chromosomes, can serve as markers of mitotic cell age and replicative potential.
To confirm that knocking down TBX2 sensitises MCF-7 cells to cisplatin as a result of an abrogated p53 response, we examined the effect of silencing p53 on markers of mitotic catastrophe in cisplatin-treated MCF-7 cells.
To test this alternative possibility, shRNA-transfected cells were synchronized at mitosis, and at 6 days post-transfection, stained for markers of mitotic spindles (α-tubulin) and centromeres (CENP-B), respectively.
Because both G2 and mitotic cells have 4N-DNA content and are not distinguishable from each other by propidium iodide staining, phosphorylation of H3-Ser10 in 4N-DNA content cells has been commonly used as a specific marker indicative of mitotic cells [ 12].
This feature has been used as a marker of cellular mitotic entry.
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