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Temperature regulation is considered a favorable surrogate marker of maturation of autonomic control in infants since it is easy to measure and minimally invasive.
To further explore this hypothesis, we analyzed the expression of KCC2, a nonspecific marker of maturation of multiple groups of interneurons and pyramidal neurons, in the cortex of control and Satb1 mutant embryos.
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Following stimulation with lipopolysaccharide (LPS) and/or cytokines, a dendritic cell line interacting with the surfaces of immunosuppressive hydrogels expressed reduced markers of maturation, including IL-12 and MHCII.
Both infected and uninfected cells upregulated phenotypic markers of maturation.
Since a reduction in markers of maturation (e.g. increased ANF and decreased calsequestrin) was also observed, Rb must have a dual role in proliferation and in maturation.
The use of markers of maturation could be very important to detect the onset of differentiation and to choose the most appropriate clinical treatment.
APCs from both groups displayed similar expression of various markers of maturation and antigen-presentation (CD40, CD80, CD86 and MHC II; Fig. 7A).
After staining, 20,000 events were acquired on a Becton Dickinson (Mountain View, CA) FACSCalibur® and the expressions of the markers of maturation were analyzed on CD11c-gated cells using CellQuest software.
Under similar conditions, immature DCs incubated with the non-lipidated HER-GP construct failed to up-regulate the DC markers of maturation and did not produce the pro-inflammatory cytokines.
A decrease of CD1a and an increase of CD83 were also examined as markers of maturation.
Batteries of developmental milestones have been designed to describe early neurodevelopment of newborn rodents and include behavioral markers of maturation of proprioception (tactile response such as grasping, placing etc), and vestibular function which involves acquisition of coordination and adequate strength [ 29, 30].
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