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Besides, the lipoperoxidation damage was only observed in K. striatum thalli (MDA, a marker of lipoperoxidation, increased significantly at 17 °C in that species) (Fig. 3).
To evaluate the extent of oxidative damage in algal cells under low temperature stress, the concentration of MDA, a marker of lipoperoxidation, was measured in the algal thalli by the method of Fraga et al. (1988).
Moreover, this marker of lipoperoxidation showed no relationship with LV systolic function.
Moreover, similar results were also obtained using complementary immunohistochemical detection of 4-HNE as another marker of lipoperoxidation.
Using a selective HPLC method, significantly increased levels of total MDA(a widely used marker of lipoperoxidation) were determined in the LV myocardium of DAU-treated animals.
The immunohistochemical detection of 4-HNE (another independent marker of lipoperoxidation) in the LV myocardium revealed a markedly higher response in the samples obtained from DAU-treated animals than in corresponding controls.
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Thiobarbituric reacting substances (TBARS) are markers of lipoperoxidation.
To assess oxidative stress in vivo, we measured urinary 8-isoprostanes, which are reliable markers of lipoperoxidation and, for the first time, sNOX2-dp, a marker of NOX2 activation by blood cells, which is a ROS generating enzyme implicated in arterial function via oxidative-stress mediated NO inactivation [ 3, 37, 38].
Two independent exploratory multivariate statistical methods (Hierarchical Tree Clustering and Principal Component Analysis) were used to identify the natural groupings of individual animals in this study with respect to the following evaluated parameters – LV cardiac function parameters, cardiac troponins and markers of apoptosis and lipoperoxidation (comprising a total of 10 variables).
As an index of lipoperoxidation, thiobarbituric acid-reactive substances (TBARS) were assayed as previously described [21].
However, this protective effect was not accompanied by any reduction of lipoperoxidation (Simunek et al, 2008).
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