We conducted sensitivity analyses to examine the robustness of our primary findings to levels of urbanicity and insurance status as markers of data consistency.
In addition, hESC-derived cells expressing high levels of SMC markers had no expression of PECAM-1 (endothelial cell marker) and α-actinin (a marker of cardiomyocytes) (data not shown) confirming again their SMC differentiation.
Similar results were obtained using active caspase 3 as a marker of apoptosis (data not shown).
The β-gal-positive cells (blue) did not colocalize with GFAP (brown), a marker of astrocytes (data not shown).
The β-gal-positive cells (blue) in the LC did not colocalize with GFAP (brown), a marker of astrocytes (data not shown).
The β-gal-positive cells (blue) did not colocalize with glial fibrillary acidic protein (GFAP) (brown), a marker of astrocytes (data not shown).
There are four main MABS variables: (i) number of backcrosses, (ii) population size, (iii) number and spacing among markers, and (iv) number of marker data points (MDPs) (calculated as per marker per plant).
DJB participated in writing the manuscript, marker development, analysis of data and co-ordination of the study.
Data was quality controlled for marker call rate, minor allele frequency and Hardy Weinburg Equilibrium (HWE): markers missing 5% of data, or with MAF of less than 2% were removed as were markers that were significantly out of HWE.
These reprogrammed cells can be expanded in ESC culture conditions, are tetraploid and express markers of pluripotency (data not shown).
