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TIM-1 is also a receptor for phosphatidylserine (PtdSer), an important marker of cells undergoing programmed cell death, or apoptosis.
Next, we cultured OPCs for 24 h in the presence of EdU (5-ethynyl-2′-deoxyuridine), a marker of cells in S-phase of the cell cycle, to determine whether the proliferative effect of growth factors was modified by cholesterol.
Using resazurin dye as a metabolic activity marker of cells, we confirmed that hypoxia had little effect on MDA-MB-231 cells, while MCF-10A and MCF-7 cells exhibited a significantly reduced metabolic activity (Fig. 1B, n = 3).
This makes CK2 not only an appealing target to counteract different kinds of tumors but also a valuable marker of cells predisposed to undergo neoplastic transformation owing to the presence in them of CK2 level exceeding a critical threshold.
PARP is one of the main cleavage targets of activated caspases and cleaved PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis [40].
This was confirmed by a 45% increase in Ki67+ cells (a marker of cells in the cell cycle) detected in Wnt5a−/− mice (Figure 4A, B).
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FDG is only a surrogate marker of cell proliferation.
Cardiac muscle is labeled in green, DNA is blue and a marker of cell division is shown in red.
Cellular ATP content was determined as a marker of cell viability using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Sweden).
The results were compared with immunoreactivity for Ki-67, a marker of cell proliferation.
It is regarded as a biochemical marker of cell-mediated immune response.
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