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Wang, W. et al. Ebf2 is a selective marker of brown and beige adipogenic precursor cells.
Ucp1 is a specific marker of brown fat [31], [32].
It is thought to be expressed exclusively in brown adipocytes, and is considered a specific marker of brown adipocytes [14].
The protein expression of uncoupling protein-1 (UCP-1), the highly specific marker of brown adipocytes, was increased 7-fold in white adipose tissue of HSL null mice compared to wildtype littermates.
Finally, protein expression of uncoupling protein 1 (UCP1), a marker of brown adipocyte differentiation, was elevated in differentiated KO and D/A cells compared with WT and was not detectable in K/R cells.
RT-PCR analysis performed on WT, PRLR KO and PRLR-rescued cell lines confirmed that expression of PRLR correlated with that of PPARγ2, a marker of adipocyte differentiation, and of UCP1, a marker of brown adipocyte lineage (Fig. 3D).
Similar(50)
Similar to markers of brown adipocyte thermogenic activity (for example, UCP1), GPR120 was preferentially expressed in mature, differentiated, brown adipocytes rather than in the stromal vascular fraction (Fig. 1c).
The expression of key markers of brown fat cell function was also investigated.
In iWAT, we observed strong evidence of GW9508-induced brownincludingupregulationlatiof of markers of brown-related thermogenesis (UCP1, PGC-1α and Sirt3), and the beige adipogenesis-related gene PRDM16 (Fig. 2b).
Burn injury results in browning of the subcutaneous fat in rodents and humans Browning occurs beyond or after 10 days post-burn injury in humans Markers of browning are reduced by the beta-blocker propranolol IL-6 is required for browning in mice post-burn injury.
Interestingly, UCP1, PGC-1α and the expression of other marker genes of brown-like adipocytes are significantly elevated in perirenal and inguinal WAT of these mice [ 44].
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