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Vidal, V. P., Ortonne, N. & Schedl, A. SOX9 expression is a general marker of basal cell carcinoma and adnexal-related neoplasms.
Keratin 14 is a specific marker of basal cells.
This appears to be a molecular marker of basal cell hyperplasia.
Immunohistochemistry for Tbr2, a marker of basal progenitor cells, demonstrated a significant increase in this progenitor population compared with wild-type (P < 0.0005, Fig. 6D F) (reference Tbr2).
In support of this, we identified an inverse correlation between miR-205 and CK14, a marker of basal cell hyperplasia and squamous restoration.
Triple immunostaining against RACK1, MITF and cytokeratin 5 (CK5), a marker of basal keratinocytes, was performed in order to better identify the cell type containing RACK1 in the epidermis.
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Interestingly, COX-2 were over-expressed in this basal-like subtype, confirming this molecule as a marker of basal-like breast tumors.
Therefore, intracellular LOXL2 is a new candidate marker of basal-like carcinomas and a target to block metastatic dissemination of this aggressive breast tumour subtype.
In summary, our present results define intracellular LOXL2 as a new marker of basal-like breast tumours associated with metastasis incidence and demonstrate its active participation in lung metastatic dissemination of basal breast carcinoma cells.
Interestingly, region 6p22.3 contains ID4, a gene long known to be a marker of basal-like breast cancers [ 20], and known to code for a DNA-binding protein that negatively regulates BRCA1 expression in breast and ovarian cancers [ 21].
Analyses of larger series of this tumor subtype will allow us to confirm the specificity of this aberration and, importantly, its possible use as a clinical marker of basal-like tumors.
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