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This makes it possible to monitor the diffusion of a marker molecule from the micropipette, through the tube, and out of the nanotube opening.
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Recently, we have reported that GD3 can be a marker molecule for isolating NSCs from mouse embryonic, postnatal and adult brains (Nakatani et al., 2010).
In addition, we assessed immunostaining for the dystrophin protein, a marker molecule for plasmatic membrane (Figure 5A), to define positive PCNA nuclei and discriminate satellite cells from other cell types.
For studying their basic biological properties and clinical applications, it is important to characterize marker molecules for identifying and isolating NSCs from brain tissues.
Nobody in the medical community finds this test to be satisfactory and the search for a marker molecule or problem protein linked to the symptoms continues.
In mouse NSCs, GD3 ganglioside is such a marker molecule.
Knowledge about specific marker molecules, which could distinguish between fibroblasts from different anatomic sites, as well as between fibroblasts at different functional or disease-related states, would massively contribute to the understanding of patho-physiological mechanisms.
This allowed direct quantification of marker molecules in a three-dimensional context.
A modified immunoassay technique allows precise quantification of converted marker molecules.
The photons arrive to voxels from nearby fluorophore molecules in a random Poisson process; likewise, the fluorophore marker molecules bind to the nearby synapses in a random Poisson process.
The expression of marker molecules was analysed by qRT PCR.
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