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In all investigated programs the free surfaces are treated by the volume-of-fluid (VOF) method and flow patterning is visualised with a scalar marker method.
Compared with the PCR-based marker method, the sequencing-based method was faster and more precise for the determination of the recombination breakpoint, achieving a resolution of 40 kb recombinant block on average (Wang et al. [2011]).
A statistical threshold corresponding to an experiment-wise type I error rate of 0.05 was established for each marker method by randomly permuting the phenotypes 1000 times.
It is the most popular RTNs-based molecular marker method at present [ 6, 13, 21].
The revertible selectable marker method was used for mutagenesis (Deng and Nickoloff 1992).
CMV was estimated using the correlation marker method and the unmeasured latent method construct (ULMC) approach.
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The total fecal collection technique was evaluated by comparing it with two different marker methods, one external (chromic oxide) and one internal (acid-insoluble ash).
Emphasis is on the obtained information of the different molecular marker methods used in mangrove genetics and on the potential to infer biogeographical patterns.
Dominant marker methods (RAPD, AFLP and ISSR) were successful to demonstrate differences in amplified DNA products at large-scale geographical distances within Avicennia species and to estimate species relationships.
Molecular marker methods using polymorphic-anonymous (that is, not of any known functional importance to the plant) markers are widely used for germplasm characterization.
We further confirmed this phenotype in the chd1Δ strain with a different resistance marker (Methods, [see Additional file 1: Figure S1A]).
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