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In this study, a flexible phase-shifting method, which does not need the additional absolute phase marker map, was developed.
Given the great expense of collecting these families and the imminent availability of a dense diallelic marker map, the families are likely to be used in allelic-association as well as linkage studies.
An additional absolute phase marker map is necessary to acquire the absolute phase with traditional phase-shifting algorithm, which not only has a low efficiency but also may not meet certain special requirements of the practical application.
MAPMAKER initially uses a maximum of eight markers, and subsequently positions additional markers within the initial (eight marker) map.
This procedure enables to determine how many peaks of similar height might arise by chance conditional on the set of phenotypes being analysed, the marker map and the family structure.
Further, this gene is physically located within 1 cM of the QTL peak as defined using both the metabolite and eQTL data with a dense marker map within this region (Figure 2) [25].
The barley mapping populations in which those QTLs were mapped were also used to build the dense integrated barley marker map used in the present study and two of those populations also to map the PERO-markers.
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Neuronal differentiation was assessed by immunostaining with β-III tubulin and the mature neuron marker, MAP-2 (Fig. 6, A and B).
An additional marker, Map-0281 on Gm02, was assigned to Gm13.
Immunostaining was performed with antibodies to neuronal marker MAP-2 (Millipore, Billerica, MA, USA) and astrocyte marker GFAP (Dako North America, Inc., Carpinteria, CA, USA).
To deeply investigate the effect of PEA on neuronal circuitry, we decided to perform analysis on a neuronal marker, MAP-2.
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