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Because the Fura-2 calcium marker leaks out of platelets that were cooled to 4°C during the preparation, the platelets were prepared with glucose and pyruvate to ensure viability at 20°C [ 7].
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This is to avoid the marker leaking onto the other side,or going all splotchy.
It is possible, however that due to the fixation step in our staining protocol, this marker may leak into the cytoplasm.
Unlike other retrograde markers that leak from the cell body after several weeks, DiI has been shown to persist in RGCs in vivo for periods of up to 9 months without fading or leakage.
This causes certain liver marker enzymes to leak out of the cell.
"While the test doesn't appear to have leaked into the public domain and can go ahead, a rogue marker did attempt to leak the test contents," the source said.
As a result, leaked markers have limited sensitivity and specificity.
The accumulation of neutrophils recovered from bronchoalveolar lavage fluid and lung homogenates was increased in aged compared with young adult mice, although bacterial outgrowth was similar in both age groups, as were markers of microvascular leak.
Vlaar et al. [64] reported that in cardiac surgery patients, RBC transfusion was associated with an increase pulmonary leak index, an early marker of acute lung injury.
Vlaar et al. [ 64] reported that in cardiac surgery patients, RBC transfusion was associated with an increase pulmonary leak index, an early marker of acute lung injury.
Mark the leak with a permanent marker once you find the source.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com