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We added two endosomal markers, an early endosomal marker FYVE (Bloomington #39695) and a later endosomal marker LAMP (Bloomington #42714).
No co-localization was observed of hMTMR4 and the early endosomal marker Rab5 [ r(13)=0.19], nor was there significant overlap in MTMR4 signal with the lysosomal marker LAMP [ r(9)=0.16] or the Golgi marker GM130 (cis-Golgi matrix protein of 130 kDa) [ r(8)=0.09] (results not shown).
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The systems have typically used either regular headlamps at half their normal brightness or special marker lamps near the headlights.
The lights are actually an amplified version of the typical taillight, and they are meant to brighten the rear marker lamps of a car so that drivers coming up from behind will notice a car sooner in foggy or hazy conditions.
Using an immunofluorescence staining technique, we assessed the colocalization of internalized bacteria with a lysosomal marker, LAMP-1.
We also examined colocalization of SBD-TMR with the more broadly distributed endolysosomal marker LAMP-GFP [81], [82].
To further examine the process of phagosome maturation, we stained the cells with the lysosome-specific marker LAMP-2 (Figure 5C).
VMR-CM-stimulated fibroblasts were then analyzed by immunofluorescence microscopy using antibodies against the lysosomal marker LAMP-1 and S100A4 (Fig. 2B).
We tried but failed to show an unquestionable full co-localization of AA/SAA reactivity and the lysosome specific marker LAMP-2 [30].
To address this, we analyzed the expression of the late phagolysosomal marker LAMP-2 (Fig. 3A) and anti-mycobacterial proteins- pp38 and iNOs (Fig. 3B, C) competitively among WT and SphK-1++ infected macrophages.
This was due to the reduced expression of major anti-mycobacterial proteins such as iNOs, p38, pp38 and late phagolysosomal marker, LAMP-2 and reduced activation of NF-kB in the infected macrophages.
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