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The SEC column was standardized using a protein marker kit (Sigma-Aldrich, P/N: MWGF1000).
Lyophilized tyrosinase (EC 1.14.18.1) from mushroom (A. bisporus) and a non-denatured protein molecular weight marker kit were purchased from Sigma-Aldrich (Steinheim, Germany).
High molecular weight native protein marker kit (GE Healthcare, Amersham-Pharmacia, 17-0445-01, usedwas used to determine the molecular mass of these complexes.
For determination of the relative molecular weight of the herein described bacterial PO from the strain A. chroococcum SBUG 1484, native PAGE using a non-denatured protein molecular weight marker kit was conducted.
Supporting this notion, Ras cells treated with cytarabine were much less likely to express the stem cell marker kit as compared to control cells (Figure 5C).
Consistent with these observations, the mRNA levels of the stem cell marker kit were lower in Ras cells and expression became virtually undetectable upon treatment with cytarabine (Figure 5C).
Similar(41)
TGI has been leveraged to build a dense tobacco genetic map [ 6] and microsatellite marker kits for variety identification [ 7].
The apparent molecular mass was determined using two molecular mass marker kits (Magic Mark, Invitrogen and Precision Plus Protein Kaleidoscope, Bio-Rad).
Among the markers, KIT showed the highest AUC (0.9).
Among all markers, KIT and miRNA146b showed the highest AUC (0.9) for malignant versus benign.
The column was calibrated with molecular weight protein standards (Gel Filtration Markers Kit MWGF1000 Sigma-Aldrich St . Louis MO, USA).
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