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The swarm marker is shown by a black square.
Where only one alphabetical marker is shown (L and M), the deformation does not move through the full simulation.
Molecular marker is shown in kDa.
Lower plot: Phase data recorded at three different cantilever-surface gap heights (only every twentieth marker is shown), the model ϕ (solid black line) and the baseline ϕ bl (dashed black line) included in the model to account for the phase response of the photothermal excitation ϕ th and the measurement electronics ϕ el.
The polymorphism information content [18] for each marker is shown in Table S1; with average PIC (0.21 for Asplenium, 0.25 for Garovaglia), lower than expected for randomly chosen bi-allelic loci (0.50) and lower than in previous DArT studies in barley (0.38) [7] and cassava (0.42) [10.42
The location of each marker is shown in Table 2 >.
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The cut-off values of cell count for positivity of each cell marker are shown in Supplementary Table S1.
Primer combination for each marker was shown on the gel images and its sequences were listed in Table 2.
The sequence of H marker was shown below: H (F: 5'-AGAATTGCTGCATGCTGTTG-3′, R: 5'-ATTCCAGCATCGATTGCTTC-3′).
For better understanding, the designing of the Gn1a-17 SNP marker and the SPIKE-01 SNP marker are shown in Additional file 2: Figure S2.
The marker was shown to be sensitive for early-stage colorectal cancers identifying 62% of Stage I/II CRCs.
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