Sentence examples for marker is integrated from inspiring English sources

The selection marker is integrated along with the edit and must be removed in a subsequent step.

We therefore generated a reporter cell line in which a neomycin-resistance marker is integrated into an endogenous PcG-repressed target locus, the Gata6 gene.

Furthermore, similar effects of CR on MET15 reporter gene expression are observed when the marker is integrated into nonsilenced regions of the genome, suggesting that the increased pigmentation observed is a metabolic effect unrelated to silencing (Smith et al., 2009).

Similarly, as the URA3 marker is integrated approximately 10 cM from the mat1 locus containing the h allele (Egel 2004), recombination between URA3 and the mat locus can produce URA3 h + haploids that would slip through the selection procedure and these would also fail to sporulate.

The progeny were subsequently also analyzed by colony PCR for the nature of the PMT2 allele present in the strains amplifying a short fragment spanning the region where the ADE2 marker was integrated (data not shown).

The sex-linked microsatellite marker was integrated on the genetic map using the same method.

As controls, the KanMX marker was integrated adjacent to the WT ERG26 and ERO1 loci of the W303 background via identical strategy, resulting in AMY1013-1A and SCVY15-2D, respectively.

The marker was integrated employing the software JoinMap 4 [ 38], using a LOD of 5.0 and a recombination frequency of 0.45.

While one additional marker was integrated from the LepR3 map Ind10-133, corresponding to Bra008931), this also co-segregated with the Rlm2 phenotype.

Previously mapped 35 simple sequence repeat markers were integrated into the PKAM map and an integrated genetic map of 996.21 cM was constructed.

CIp20-based plasmids, which contain both URA3 and HIS1 markers, were integrated at the RPS1 locus, and correct integration confirmed by PCR diagnosis (Dennison et al., 2005).