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No marker is available to predict this progression.
This marker is available to introduce Gn1a-Habataki allele (Type 3) into both Gn1a-Type 1 and Gn1a-Type 2 backgrounds.
Except for Parao, all genotypes showed the same band size as Habataki (Fig. 1b bottom), indicating that this marker is available only for Gn1a MAS in a Parao background.
If no marker is available within this range, a next marker is randomly selected among the next Hop/2 markers and the information is written on it, by overwriting the oldest entry, if needed.
This marker was tested in the parental varieties, and the result was consistent with the Gn1a-17 SNP marker analysis, except for Parao (Fig. 1b middle), indicating that the marker is available to introduce Gn1a-Habataki allele into Gn1a-Type 2 backgrounds but not Parao background.
For eight microsatellite markers, namely Hd46, SAPD00344, Did12, AJ418596, AJ418568, AF478569, SAPD00688 and SAPD00677 located in the region of interest, BAC clones were sequenced, thus genomic information around each marker is available for SNP (Single Nucleotide Polymorphism) detection (Fig. S1).
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Sequences, PCR primers, and details of the SNP assay for each marker are available on the Ambystoma Research Network website.
A suite of microsatellite markers is available for T. dalmanni [54] that allows paternity to be assigned to offspring [39].
More information concerning these wheat microsatellite markers is available in the GrainGenes 2.0 database [http://wheat.usda.gov/GG2/index.shtml].
A linkage group of eight markers is available.
Information regarding Mt-FTQTL6-linked markers is available in Additional file 1.
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