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The resultant amplicon was co-transformed with pREP3X plasmid, carrying a LEU2 selection marker, into a flag-mmi1 (wild-type mmi1) strain.
Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris.
Firstly, we may ask what we gain from introducing a marker into a study.
Yakubov et al. [ 34] converted the RAPD marker into a Sequenced Characterized Amplified Region (SCAR) marker; however, amplification of the SCAR marker was observed in both sexes.
Inclusion of this marker into a dataset of microsatellite markers from this population, and using JoinMap 3.0 to group and order markers, linked the gene to linkage group 20.
Therefore, we directly tested mutation position biases by introducing the counterselectable URA3 marker into a subset of mutator lines at loci increasing in distance from the Tel VI-L.
Similar(53)
FRAP involves observing the rate of recovery of fluorescence resulting from the movement of a fluorescent marker into an area which contains the same marker that has been rendered nonfluorescent via an intense photobleaching pulse of laser light (Fig. 4d).
In this paper, we provide a primer for drug development teams who are eager to implement a predictive patient segmentation marker into an early clinical trial in a way that facilitates subsequent development of a companion diagnostic.
Incorporation of such markers into a biomarker panel may improve diagnostic specificity.
We next asked whether combining both transcripts and markers into a single prediction algorithm would improve our ability to predict SMP response.
In addition to independent analyses of each marker, a mitochondrial (12S+16S+COIII) and a nuclear (18S+28S) data set, we combined all five markers into a simultaneous analysis of mitochondrial and nuclear sequences.
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