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Herein, we demonstrate a highly sensitive and specific pentaplex PCR assay that requires one-time optimization of quasi-monomorphic variation range (QMVR) for each marker in normal DNA.
These results suggest that besides as a marker in normal and abnormal mesenchymal cells [2], [34], [35], vimentin has a novel role in connection with AFP+ cells and BrdU+ cells, indicating these cells are activated for proliferation.
Although the five mono-repeat markers in pentaplex PCR have been suggested to be highly monomorphic in germline DNA from a wide spectrum of populations worldwide, the success of this MSI assay heavily relies on the accurate determination of QMVR for each marker in normal DNA [11].
Moreover, TTG-2 Rhombotin-2 is a cysteine rich protein with LIM motif and immunohistologic analysis show that LMO2 protein is expressed as a nuclear marker in normal germinal-center (GC) B cells and GC-derived B-cell lines and in a subset of GC-derived B-cell lymphomas [56].
The genotype distribution and allele frequencies of the (G-T n microsatellite marker in normal control populations and patient populations are summarised in table 1.
K-14 is a ME marker in normal tissue, whereas in transformed tissues it is regarded as a "proliferation" antigen (Fig. 4A,B).
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Volkow, N. D. et al. Parallel loss of presynaptic and postsynaptic dopamine markers in normal aging.
Since circulating CD133+ stem cells did not differentiate spontaneously into myotubes in vitro [16], we investigated the expression of human myogenic markers in normal and dystrophic sorted CD133+CXCR4+CD34- cells co-cultured with C2C12 murine myoblasts.
Representative photomicrographs show the endothelial markers in normal skin and c-GVHD skin are quite visible (Figure 4 A-C) whereas VE cadherin is missing from vessels in SSc skin (Figure 4 D).
Two cases (n°53 and 99, Table 5), with homozygous markers in normal tissue specimen, presented one allele with a deletion of some base-pairs in tumor tissue specimen and were considered as informative cases with allelic mutation.
Additionally, the biodistribution of both markers in normal tissues was assessed under similar conditions.
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