After removing small regions and implementing region filling, the internal marker image is obtained.
The cross-shaped markers were printed with the same radioactive ink as the printout and were visible on the PET image obtained.
Secondly, we used a marker-based watershed approach [ 20] to get an initial segmentation of the neurons from the MGRF-relaxed image, obtained in the first step.
Image obtained from the robot's camera.
Image obtained using colorized transmission electron microscopy.
To quantify the fluorescence intensity of the markers of interest, images obtained from CellSearch or confocal microscopy were subjected to java-based image processing with the use of ImageJ program (NIH).
Images obtained were 400x400 microns wide.
The binary internal marker image B in (x, y) is obtained by implementing region filling on B ′ x, y).
The external marker image B ex (x, y) is obtained by implementing morphological operations such as thinning and skeleton extracting on the binary image.
Figure 3 Minima imposition technique: (a) input image g and marker image f m ; (b) the modified edge strength image g ′ obtained from the reconstruction by erosion.
