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We presented a new method for marker gene detection using microarray gene expression data.
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A real-time reverse transcriptase polymerase chain reaction (RT-PCR) screening study has demonstrated the specific overexpression of the HABP2 gene in lung adenocarcinoma, among six candidate marker genes for detection of non-small cell lung cancer [39].
Amplifications of the bcsp31 (224 bp) and IS711 (350 bp) genes were observed, which are universally accepted marker genes for detection of Brucella spp. (Figs. 1 and 2).
Data from lineage tracing experiments, expression patterns of neural crest cell (NCC) marker genes and detection of apoptotic cells indicate that the malformation of bones in Prtg-deficient mice is due to increased apoptosis of rostral CNCCs (R-CNCCs).
This may be of particular relevance if Wolbachia surveys are based on a single or a few marker genes for detection of infections in a host species, and without a broader sampling effort from the host's distribution (also see [ 75]).
This observation suggests that PIWIL1 could be a marker gene for the detection of ES cell-derived germ cells.
Suppression of marker gene expression makes detection of insertions in such regions nearly impossible.
A real-time PCR was performed for all cDNA dilutions, for each marker gene individually, and the detection limit of the assay was defined.
For the single marker test SM, we counted a gene detection for any gene containing a marker below the P-value threshold.
In addition, the availability of sensitive techniques to monitor gene expression, including detection of reporter gene expression using antibodies and detection of endogenous marker gene expression using quantitative RT-PCR, provides an effective means for comparing wild-type and mutant retinas from genetically engineered mice.
Both functional marker genes allowed the detection of representatives belonging to the Desulfobulbaceae, Desulfobacteraceae, and Desulfomicrobiaceae families.
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