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Thus, it is difficult to use these trivalent As metabolites as a marker for this study.
While electrophoretic mobility was used purely as a convenient phenotypic marker for this study, the traits underlying electrophoretic mobility in viruses are very likely of evolutionary significance.
Among these, the AphVIII gene from Streptomyces rimosus coding for an aminoglycoside 3'-phosphotransferase, which confers resistance to the antibiotic paromomycin [ 31], was used as a selection marker for this study because the drug-resistant phenotype is stable [ 32].
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Vascular markers for this study included the endothelial cell markers CD31 and CD105, as well as smooth muscle alpha-actin (α-SMA).
To identify anchor markers for this study, we first identified a set of SNP markers, which we used to assign the 19 grapevine chromosomes to 19 linkage groups (LGs).
To conserve markers for this study, the KanMX4 cassette in strain YO528 was replaced with NatMX4 using EcoRI-linearized p4339 (Tong et al. 2001) (gift of Charles Boone), generating strain YO794.
Like with other screening studies, it is certainly premature to delineate direct candidates for novel antidepressant targets or for diagnostic markers from this study.
Whereas it is generally known that resistance to rifampicin is a surrogate marker for MDRTB, this study observed a high rate of monoresistance to rifampicin.
Most PCR based DNA markers used for this study have already been published: Markers with an asterisk in Fig. 2 were designed by RGP (http://rgp.dna.affrc.go.jp/E/publicdata/caps/index.html).html
Differences in amplification, sequencing and polymorphism rates among different types of markers used for this study reflect the mode of design of the markers.
The PCR conditions for indel and SSR markers used for this study were 95°C for 10 min, 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s with subsequent final extension of 72°C for 1 min.
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