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van der Flier, L. G., Haegebarth, A., Stange, D. E., van de Wetering, M. & Clevers, H. OLFM4 is a robust marker for stem cells in human intestine and marks a subset of colorectal cancer cells.
Two recent studies identified CD133 as a marker for stem cells in colorectal cancers (CRC) [8], [9].
p63, another marker for stem cells or basal cells, was used here as the positive control.
It was originally regarded as a marker for stem and progenitor cells of the hematopoietic system [ 31, 32].
Recently, GD2, a disialoganglioside has been identified as a marker for stem cells of MSCs [ 10] and breast cancer [ 11].
We and several other labs have shown that it is a marker for stem cells in many tissues.
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Zhu F, Qian W, Zhang H, Liang Y, Wu M, Zhang Y. et al. SOX2 is a marker for stem-like tumor cells in bladder cancer.
The PAX7 protein is specific for satellite stem cells in skeletal muscle and has previously been used as marker for stem-cell count in muscle24.
However, CD133, a controversial marker for stem-like cancer cells, was expressed at low to intermediate levels in two out of three xenografts of either phenotype (Fig. S2).
However, the true identity of these cells remains obscure largely due to the lack of specific bona fide antigenic markers for stem versus progenitor cells.
Therefore, the development of genetic markers for stem cell identification might constitute a valuable tool for improving the efficiency of GCT.
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