To date, there is little work performed in glioma focused on finding markers for early tumour detection (Liu et al, 2005), and in the general field of oncology there are only a few studies on finding markers for quantification of treatment efficacy (Gadducci et al, 2004; Reyzer et al, 2004).
In this study, we confirmed and extended our previous observation and hypothesis, as leukemic ITD+ DC did not show expression of molecules used routinely as markers for quantification of differentiated myeloid or plasmacytoid DC in peripheral blood (BDCA-1, BDCA-2, and BDCA-3).
Multiplex IF staining with cytokeratin (epithelial marker) allowed for quantification of Src and ezrin expressions in the epithelial compartment and exclusion of the stroma region.
We used these marker sets for quantification of MPCs by flow cytometric analysis in the original cell population and after in vitro cultivation.
It can therefore be used as a reliable marker for the quantification of adult neurogenesis [27], [28].
Epithelial cell adhesion molecule (EpCAM) has been used as surrogate marker for the quantification of circulating tumour cells (CTC).
For the current study, we used again synaptophysin immunohistochemistry as an established marker for the quantification of the synaptic density in different regions of the cerebral cortex, the striatum, and the hippocampus.
These bodies provide a useful fluorescent marker for lipofuscin quantification in the living eye, which can serve as long-lasting evidence of excessive past accumulation of all-trans-retinal.
Alizarin red staining is famously known as a marker for the quantification of calcium and evaluation of differentiation [ 16, 22]. Figure 10 depicts the pristine PLGA, nHA/PLGA, and BMP-g-nHA/PLGA hybrid nanofiber scaffolds stained with alizarin red after 15 days' culture.
