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PCNA, a marker for proliferating cells, was negatively correlated with MeHg dose, with a significant reduction in cell number in the forebrain and spinal cord of exposed embryos by tadpole stages.
Because of this absence of the antigen in resting cells, it is a good marker for proliferating cells [18].
We therefore analyzed thymocytes from wt and ADAM8−/− mice for the expression of Ki-67, a marker for proliferating cells [28], and activated Caspase-3, an apoptosis marker [29].
We found that GFP+ cells expressed neither GFAP (Figure 4a-a″,f), a marker for glial cells and neuronal stem cells, nor Ki67, a marker for proliferating cells, indicating that these GFP+ cells are postmitotic (Figure 4b-b″,f).
To support this result, expression of PCNA, a marker for proliferating cells, and cell cycle were analysed in H69 cells.
Further interesting data were related to the modulation of the PCNA expression, as a marker for proliferating cells.
Similar(40)
To address this we stained hippocampal sections taken from 33-week old HD and WT animals, which had received BrdU injections, with markers for proliferating cells (BrdU), newly born neurons (DCX), and astrocytes/stem cells (GFAP).
The ob/ob mice showed an increase in the proliferating cell nuclear antigen (PCNA) (P = 0.010), a marker molecule for proliferating satellite cells [24], [25], and a decrease in cyclin D1 (P = 0.0001) proteins (Figure 4B).
Fourteen markers were found for proliferating calli and 35 markers for calli at the end of the embryogenesis induction phase.
These markers are suitable for proliferating endothelium giving none or poor staining of lymphatics and normal quiescent blood vessels.
Since H3KT identifies mitotic figures in any proliferating cell, the type of cell with mitosis may be further identified with incorporation of another IHC marker (eg, Mart-1/H3KT for proliferating melanocytes or CK20/H3KT for proliferating Merkel cells).
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