Furthermore, a positive staining for nitrotyrosine, a marker of nitrosative injury, was mainly observed in mononuclear cells infiltrated in the colonic mucosa.
On day 35, a positive staining for nitrotyrosine, a marker of nitrosative injury, was found in the joints of vehicle-treated CIA-control mice.
The aim of the present study was to investigate the effects of levosimendan on markers for diaphragm nitrosative and oxidative stress, inflammation and proteolysis in a mouse model of endotoxemia and mechanical ventilation.
Nitrotyrosine formation (a marker of nitrosative stress/peroxynitrite formation) was only detected in the atheromatous plaque and was 80% more abundant (p = 0.056) in the CA of septic animals in contrast to sham (Figs. 1a and 2).
Twenty-four hours after SCI, nitrotyrosine, a specific marker of nitrosative stress, was measured by immunohistochemical analysis in the spinal cord sections to determine the localization of "peroxynitrite formation" and/or other nitrogen derivatives produced during SCI.
Thus, NO2-Tyr can be used as a general marker of nitrosative stress.
Serum nitrotyrosine abundance (a marker of nitrosative stress) was measured by ELISA.
3-Nitrotyrosine expression, a marker of nitrosative damage, was significantly elevated in pulmonary arteries from hypoxic rats compared to NX, being significantly larger in CH compared to CIH2x2).
Nitrosylation of cell permeable DAF-2DA (4,5-diaminofluorescein-diacetate) (Sigma) was used as a marker of nitrosative stress and N2O3 formation as previously shown [ 22].
There were positive correlations between markers of nitrosative stress and inflammatory mediators in sputum.
